MicroRNA-139-3p regulates osteoblast differentiation and apoptosis by targeting ELK1 and interacting with long noncoding RNA ODSM

• DOI: 10.1038/s41419-018-1153-1
• Publication Date: 2018-10-31T08:43:17Z
• Authors: Yixuan Wang; Ke Wang; Zebing Hu; Hua Zhou; Lijun Zhang; Han Wang; Gaozhi Li; Shu Zhang; Xinsheng Cao; Fei Shi
• Keywords: Male; 0301 basic medicine; Oligoribonucleotides; Osteoblasts; Base Sequence; Caspase 3; Primary Cell Culture; Apoptosis; Cell Differentiation; Mechanotransduction, Cellular; Article; Cell Line; Mice, Inbred C57BL; Mice; MicroRNAs; 03 medical and health sciences; Gene Expression Regulation; Hindlimb Suspension; Proto-Oncogene Proteins c-bcl-2; Osteogenesis; Animals; RNA, Long Noncoding; Femur

Abstract Recent studies have confirmed that microRNAs and lncRNAs can affect bone cell differentiation formation. In this study, miR-139-3p was upregulated in the femurs of hindlimb unloading mice MC3T3-E1 cells under simulated microgravity; effect related to osteoblast apoptosis. Silencing attenuated suppression promotion apoptosis induced by microgravity. ELK1 is a target essential for regulate An differentiation-related lncRNA could interact with (lncRNA ODSM) identified Further investigations demonstrated ODSM promote differentiation. Therefore, research first reveal critical role ODSM/miR-139-3p/ELK1 pathway osteoblasts, these findings suggest potential value osteoporosis diagnosis therapy.