NSUN2 modified by SUMO-2/3 promotes gastric cancer progression and regulates mRNA m5C methylation

• DOI: 10.1038/s41419-021-04127-3
• Publication Date: 2021-09-09T13:03:56Z
• Authors: Yuanbo Hu; Chenbin Chen; Xinya Tong; Sian Chen; Xianjing Hu; Bujian Pan; Xiangwei Sun; Zhiyuan Chen; Xinyu Shi; Yingying Hu; Xian Shen; Xiangyang Xue; Mingdong Lu
• Keywords: Male; 0301 basic medicine; Carcinogenesis; Methylation; Models, Biological; Article; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; Neoplasm Invasiveness; Amino Acid Sequence; Aged; Cell Proliferation; Cell Nucleus; QH573-671; Base Sequence; Methyltransferases; Middle Aged; 3. Good health; Gene Expression Regulation, Neoplastic; Mutation; 5-Methylcytosine; Disease Progression; Female; Cytology

Abstract The 5-methylcytosine (m5C) RNA methyltransferase NSUN2 is involved in the regulation of cell proliferation and metastasis formation upregulated multiple cancers. However, biological significance gastric cancer (GC) modification itself have not been fully investigated. Here, we analyzed expression level tissue microarrays containing 403 GC tissues by immunohistochemistry. was GC, that it a predictor poor prognosis. promotes proliferation, migration, invasion cells vitro. We also demonstrated small ubiquitin-like modifier (SUMO)-2/3 interacts directly with stabilizing mediating its nuclear transport. This facilitates carcinogenic activity NSUN2. Furthermore, m5C bisulfite sequencing (Bis-seq) NSUN2-deficient showed m5C-methylated genes are cancer-related signaling pathways. PIK3R1 PCYT1A may be target participate progression. Our findings revealed novel mechanism which functions provide new treatment options for patients.