ZMYND8 suppresses MAPT213 LncRNA transcription to promote neuronal differentiation

• DOI: 10.1038/s41419-022-05212-x
• Publication Date: 2022-09-05T15:03:05Z
• Authors: Santanu Adhikary; Vipin Singh; Ramesh Choudhari; Barbara Yang; Swagata Adhikari; Enrique I. Ramos; Soumi Chaudhuri; Siddhartha Roy; Shrikanth S. Gadad; Chandrima Das
• Keywords: Ectopic expression; Transcription

Abstract Zinc Finger transcription factors are crucial in modulating various cellular processes, including differentiation. Chromatin reader MYND (Myeloid, Nervy, and DEAF-1) type containing 8 (ZMYND8), an All-Trans Retinoic Acid (ATRA)-responsive gene, was previously shown to play a role promoting the expression of neuronal-lineage committed genes. Here, we report that ZMYND8 promotes neuronal differentiation by positively regulating canonical MAPT protein-coding gene isoform, key player axonal development neurons. Additionally, modulates gene-isoform switching epigenetically silencing regulatory regions within thereby suppressing non-protein-coding isoforms such as MAPT213 . Genetic deletion led increase potentially suppressed parental transcript related programs. In addition, ectopic repression transcript. Similarly, ZMYND8-driven regulation also observed other differentiation-promoting Collectively our results elucidate novel mechanism ZMYND8-dependent different lineage committing genes, , promote neural