The accumulation of miR-125b-5p is indispensable for efficient erythroblast enucleation

• DOI: 10.1038/s41419-022-05331-5
• Publication Date: 2022-10-21T09:02:48Z
• Authors: Fang Fang; Lei Xu; Liqing Liang; Mingyi Qu; Hailei Yao; Wen Yue; Lin Chen; Dongli Chen; Zeng Fan; Lijuan He; Xue Nan; Huanhuan Zhang; Xiaoyan Xie; Xuetao Pei
• Keywords: Erythroblast

Abstract Erythroblast enucleation is a precisely regulated but not clearly understood process. Polycythemia shows pathological erythroblast enucleation, and we discovered low miR-125b-5p level in terminal erythroblasts of patients with polycythemia vera (PV) compared to those healthy controls. Exogenous upregulation levels restored the rate normal levels. Direct downregulation mouse simulated issue found PV, accumulation was enucleating erythroblasts, collectively suggesting importance for enucleation. To elucidate role gain- loss-of-function studies were performed. Overexpression improved erythroleukemia cells primary erythroblasts. Infused higher also exhibited accelerated In contrast, inhibitors significantly suppressed erythrocyte Intracellular imaging revealed that addition cytoskeletal assembly nuclear condensation, overexpression resulted mitochondrial reduction depolarization. Real-time PCR, western blot analysis, luciferase reporter assays, small molecule inhibitor supplementation gene rescue assays Bcl-2, as direct target miR-125b-5p, one key mediators during Following suppression activation caspase-3 subsequent ROCK-1 rearrangement conclusion, this study first reveal pivotal