A virus-like particle-based tetravalent vaccine for hand, foot, and mouth disease elicits broad and balanced protective immunity

• DOI: 10.1038/s41426-018-0094-1
• Publication Date: 2018-05-18T17:26:18Z
• Authors: Wei Zhang; Wenlong Dai; Chao Zhang; Yu Zhou; Pei Xiong; Shuxia Wang; Xiaohua Ye; Qingwei Liu; Dongming Zhou; Zhong Huang
• Keywords: Virus-like particle; Enterovirus 71; Coxsackievirus

Hand, foot, and mouth disease (HFMD) is an infectious that mainly affects infants children, causing considerable morbidity mortality worldwide. HFMD commonly caused by enterovirus 71 (EV71) coxsackieviruses A16 (CVA16), A6 (CVA6), A10 (CVA10). Formalin-inactivated EV71 vaccines are currently available in China; however, these fail to confer cross-protection against infections other HFMD-causing enteroviruses, highlighting the necessity of developing a multivalent vaccine. Our previous studies demonstrated recombinant virus-like particles (VLP) EV71, CVA16, CVA6 capable inducing protective immunity homologous virus challenges mice. In this study, we generated CVA10-VLP using baculovirus-insect cell expression system then combined with EV71-VLP, CVA16-VLP, CVA6-VLP formulate tetravalent VLP Immunogenicity efficacy vaccine was compared monovalent vaccines. Mouse immunization revealed elicited antigen-specific long-lasting serum antibody responses comparable those its corresponding Moreover, immune sera strongly neutralized CVA10, strains neutralization titers similar their counterparts, indicating good compatibility among four antigens combination Importantly, passively transferred vaccine-immunized conferred efficient protection single or mixed viruses mice, whereas could only protect mice homotypic but not heterotypic challenges. These results demonstrate represents promising broad-spectrum candidate.