Landscape of the genome and host cell response of Mycobacterium shigaense reveals pathogenic features

• DOI: 10.1038/s41426-018-0116-z
• Publication Date: 2018-06-22T13:46:40Z
• Authors: Haiqin Jiang; Jiya Sun; Yanqing Chen; Zhiming Chen; Le Wang; Wei Gao; Ying Shi; Wenyue Zhang; Youming Mei; Santosh Chokkakula; Varalakshmi Vissa; Taijiao Jiang; Aiping Wu; Hongsheng Wang
• Keywords: CXCL5

A systems approach was used to explore the genome and transcriptome of Mycobacterium shigaense, a new opportunistic pathogen isolated from patient with skin infection, host response assessed using macrophage infection model. The M. shigaense comprises 5,207,883 bp, 67.2% G+C content 5098 predicted coding genes. Evolutionarily, bacterium belongs cluster in phylogenetic tree along three target pathogenic strains, namely, avium, triplex simiae. Potential virulence genes are indeed expressed by under culture conditions. Phenotypically, had similar replication capacities model as species compared tuberculosis. activated NF-κB, TNF, cytokines chemokines innate immune-related signaling pathways elicited an early shared bacilli except upregulated specific such TLR7, CCL4 CXCL5. We performed integrated comparative analysis shigaense. Multigroup comparison indicated certain differences typical terms gene features response.