Ultrasensitive reversible chromophore reaction of BODIPY functions as high ratio double turn on probe

• DOI: 10.1038/s41467-017-02270-0
• Publication Date: 2018-01-18T20:24:19Z
• Authors: Dehui Hu; Tao Zhang; Shayu Li; Tianjun Yu; Xiaohui Zhang; Rui Hu; Jiao Feng; Shuangqing Wang; Tongling Liang; Jianming Chen; Lyubov N. Sobenina; Boris A. Trofimov; Yi Li; Jinshi Ma; Guoqiang Yang
• Keywords: Chromophore; BODIPY

Chromophore reactions with changes to conjugation degree, especially those between the conjugated and unconjugated state, will bring a large spectral variation. To realize such process, meso-naked BODIPY (MNBOD) two electron-withdrawing groups around core is designed synthesized. The resulting system extremely sensitive bases. red, highly fluorescent solution readily becomes colorless non-fluorescent after base addition; however, color fluorescence can be totally instantly restored by addition of acid or formaldehyde. Analyses show that identical MNBODs are connected C-C single bond (sp3) at meso-position through radical reaction results in an unconjugated, dimer complexed When bases consumed, immediately dissociates into fluorescent, MNBOD monomer. With 260 nm change over 120,000 turn-on ratio, this chromophore-reaction utilized as reaction-based dual-signal probe.