Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome

• DOI: 10.1038/s41467-018-03143-w
• Publication Date: 2018-05-10T09:18:43Z
• Authors: B. A. Blount; G-O. F. Gowers; J. C. H. Ho; R. Ledesma-Amaro; D. Jovicevic; R. M. McKiernan; Z. X. Xie; B. Z. Li; Y. J. Yuan; T. Ellis
• Keywords: Synthetic Biology; Metabolic Engineering; Heterologous

Synthetic biology tools, such as modular parts and combinatorial DNA assembly, are routinely used to optimise the productivity of heterologous metabolic pathways for biosynthesis or substrate utilisation, yet it is well established that host strain background just important determining productivity. Here we report in vivo genomic rearrangement Saccharomyces cerevisiae yeast with a synthetic chromosome V can rapidly generate new, improved strains genetic backgrounds favourable diverse pathways, including those violacein penicillin xylose utilisation. We show how chromosomes by SCRaMbLE be easily determined using long-read nanopore sequencing explore experimental conditions diversification screening. This genome approach engineering provides improvements fast, simple accessible way, making valuable addition existing improvement techniques.