Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation

• DOI: 10.1038/s41467-018-03641-x
• Publication Date: 2018-04-04T02:51:11Z
• Authors: Gaëlle Marteil; Adan Guerrero; André F. Vieira; Bernardo P. de Al...; Pedro Machado; Susana Mendonça; Marta Mesquita; Beth Villarreal; Irina Fonseca; Maria E. Francia; Katharina Dores; Nuno P. Martins; Swadhin C. Jana; Erin M. Tranfield; Nuno L. Barbosa-M...; Joana Paredes; David Pellman; Susana A. Godinho; Mónica Bettencour...
• Keywords: Centriole; Chromosome instability

Abstract Centrosomes are the major microtubule organising centres of animal cells. Deregulation in their number occurs cancer and was shown to trigger tumorigenesis mice. However, incidence, consequence origins this abnormality poorly understood. Here, we screened NCI-60 panel human cell lines systematically analyse centriole structure. Our screen shows that amplification is widespread highly prevalent aggressive breast carcinomas. Moreover, identify another recurrent feature cells: size deregulation. Further experiments demonstrate severe over-elongation can promote through both fragmentation ectopic procentriole formation. Furthermore, show overly long centrioles form over-active centrosomes nucleate more microtubules, a known cause invasiveness, perturb chromosome segregation. establishes deregulation as features cells identifies novel causes consequences those abnormalities.