In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs

• DOI: 10.1038/s41467-018-04652-4
• Publication Date: 2018-05-31T13:31:54Z
• Authors: Mo Li; Mengxi Zheng; Siyu Wu; Cheng Tian; Di Liu; Yossi Weizmann; Wen Jiang; Guansong Wang; Chengde Mao
• Keywords: Nucleic acid structure

Programmed self-assembly of nucleic acids is a powerful approach for nano-constructions. The assembled nanostructures have been explored various applications. However, acid assembly often requires chemical or in vitro enzymatical synthesis DNA RNA, which not cost-effective production method on large scale. In addition, the difficulty cellular delivery limits vivo Herein we report strategy that mimics protein production. Gene-encoded duplexes are transcribed into single-stranded RNAs, self-fold well-defined RNA same way as polypeptide chains fold proteins. resulting nanostructure contains only one component molecule. This allows both and nanostructures. synthesized strands can designed inside cells. work suggests to synthesize scale at low cost but also facilitates