A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma

• DOI: 10.1038/s41467-018-06862-2
• Publication Date: 2018-10-22T13:27:10Z
• Authors: Maolei Zhang; Kun Zhao; Xiaoping Xu; Yibing Yang; Sheng Yan; Ping Wei; Hui Liu; Jianbo Xu; Feizhe Xiao; Huangkai Zhou; Xuesong Yang; Nunu Huang; Jinglei Liu; Kejun He; Keping Xie; Gong Zhang; Suyun Huang; Nu Zhang
• Keywords: Circular RNA

Circular RNAs (circRNAs) are a large class of transcripts in the mammalian genome. Although translation circRNAs was reported, additional coding and functions their translated products remain elusive. Here, we demonstrate that an endogenous circRNA generated from long noncoding RNA encodes regulatory peptides. Through ribosome nascent-chain complex-bound sequencing (RNC-seq), discover several peptides potentially encoded by circRNAs. We identify 87-amino-acid peptide circular form intergenic non-protein-coding p53-induced transcript (LINC-PINT) suppresses glioblastoma cell proliferation vitro vivo. This directly interacts with polymerase associated factor complex (PAF1c) inhibits transcriptional elongation multiple oncogenes. The expression this its corresponding decreased compared levels normal tissues. Our results establish existence potential tumorigenesis.