Arabidopsis AGDP1 links H3K9me2 to DNA methylation in heterochromatin

• DOI: 10.1038/s41467-018-06965-w
• Publication Date: 2018-10-25T11:50:14Z
• Authors: Cuijun Zhang; Xuan Du; Kai Tang; Zhenlin Yang; Li Pan; Peipei Zhu; Jinyan Luo; Yuwei Jiang; Hui Zhang; Huafang Wan; Xingang Wang; Fengkai Wu; W. Andy Tao; Xin-Jian He; Heng Zhang; Ray A. Bressan; Jiamu Du; Jian-Kang Zhu
• Keywords: RNA-Directed DNA Methylation; Heterochromatin protein 1

Abstract Heterochromatin is a tightly packed form of chromatin that associated with DNA methylation and histone 3 lysine 9 (H3K9me). Here, we identify an H3K9me2-binding protein, Agenet domain (AGD)-containing p1 (AGDP1), in Arabidopsis thaliana . Here find AGDP1 can specifically recognize the H3K9me2 mark by its three pairs tandem AGDs. We determine crystal structure 1 2 cassette (AGD12) Raphanus sativus complex peptide. In complex, peptide adopts unique helical conformation. AGD12 recognizes H3K4me0 marks hydrogen bonding hydrophobic interactions. addition, required for transcriptional silencing, non-CG methylation, H3K9 dimethylation at some loci. ChIP-seq data show preferentially occupies long transposons heterochromatin marks. Our findings suggest that, as heterochromatin-binding links to regions.