BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitment

• DOI: 10.1038/s41467-018-07799-2
• Publication Date: 2018-12-12T17:20:45Z
• Authors: Giuseppina D’Ales...; Donna Rose Whelan; Sean Michael Howard; Valerio Vitelli; Xavier Renaudin; Marek Adamowicz; Fabio Iannelli; Corey Winston Jon...; MiYoung Lee; Valentina Matti; Wei Ting C. Lee; Michael John Morten; Ashok Raraakrishn...; Petr Cejka; Eli Rothenberg; Fabrizio d’Adda d...
• Keywords: RNase H

DNA double-strand breaks (DSBs) are toxic lesions, which, if not properly repaired, may lead to genomic instability, cell death and senescence. Damage-induced long non-coding RNAs (dilncRNAs) transcribed from broken ends contribute damage response (DDR) signaling. Here we show that dilncRNAs play a role in DSB repair by homologous recombination (HR) contributing the recruitment of HR proteins BRCA1, BRCA2, RAD51, without affecting DNA-end resection. In S/G2-phase cells, pair resected form DNA:RNA hybrids, which recognized BRCA1. We also BRCA2 directly interacts with RNase H2, mediates its localization DSBs S/G2 cell-cycle phase, controls hybrid levels at DSBs. These results demonstrate regulated HR-mediated repair.