Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly

• DOI: 10.1038/s41467-018-08028-6
• Publication Date: 2019-01-02T12:24:47Z
• Authors: Shen Wang; Yun Li; Jihong Gong; Sheng Ye; Xiaofei Yang; Rongguang Zhang; Cong Ma
• Keywords: Synaptobrevin; SNARE complex

The transition of the Munc18-1/syntaxin-1 complex to SNARE complex, a key step involved in exocytosis, is regulated by Munc13-1, SNAP-25 and synaptobrevin-2, but underlying mechanism remains elusive. Here, we identify an interaction between Munc13-1 membrane-proximal linker region reveal its essential role exocytosis. Upon this interaction, not only recruits synaptobrevin-2-embedded vesicles target membrane also renders synaptobrevin-2 motif more accessible complex. Afterward, entry leads half-zippered assembly, which eventually dissociates complete formation. Our data suggest that Munc18-1 together serve as functional template orchestrate assembly.