Development of an immunodeficient pig model allowing long-term accommodation of artificial human vascular tubes

• DOI: 10.1038/s41467-019-10107-1
• Publication Date: 2019-05-21T10:11:42Z
• Authors: Manabu Itoh; Yosuke Mukae; Takahiro Kitsuka; Kenichi Arai; Anna Nakamura; Kazuyoshi Uchihashi; Shuji Toda; Kumika Matsubayashi; Jun-ichi Oyama; Koichi Node; Daisuke Kami; Satoshi Gojo; Shigeki Morita; Takahiro Nishida; Koichi Nakayama; Eiji Kobayashi
• Keywords: Animal model

Abstract Before they are used in the clinical setting, effectiveness of artificially produced human-derived tissue-engineered medical products should be verified an immunodeficient animal model, such as severe combined mice. However, small models not sufficient to evaluate large-sized for human use. Thus, large model is necessary order properly efficacy products, artificial grafts. Here we report development pig operational (OIDP), by surgically removing thymus and spleen, creating a controlled immunosuppressive protocol using combination drugs commonly setting. We find that this allows long-term accommodation vascular The OIDP essential step towards comprehensive clinically relevant evaluation cell regeneration strategies at preclinical stage.