An anionic human protein mediates cationic liposome delivery of genome editing proteins into mammalian cells

• DOI: 10.1038/s41467-019-10828-3
• Publication Date: 2019-07-02T10:02:56Z
• Authors: Y. Bill Kim; Kevin T. Zhao; David B. Thompson; David R. Liu
• Keywords: Cationic polymerization

Delivery into mammalian cells remains a significant challenge for many applications of proteins as research tools and therapeutics. We recently reported that the fusion cargo to supernegatively charged (-30)GFP enhances encapsulation by cationic lipids delivery cells. To discover polyanionic with optimal properties, we evaluate negatively natural human their ability deliver cultured primary fibroblasts. Here ProTα, small, widely expressed, intrinsically disordered protein, enables up ~10-fold more efficient lipid-mediated protein compared (-30)GFP. ProTα at low- mid-nM concentrations two unrelated genome editing proteins, Cre recombinase zinc-finger nucleases, under conditions in which or lipid alone does not result substantial activity. may enable cell when potency is limiting.