A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

• DOI: 10.1038/s41467-019-11648-1
• Publication Date: 2019-08-14T10:02:36Z
• Authors: Mindong Liang; Zilong Li; Weishan Wang; Jiakun Liu; Leshi Liu; Guoliang Zhu; Loganathan Karthik; Man Wang; Ke-Feng Wang; Zhong Wang; Jing Yu; Yuting Shuai; Jiaming Yu; Lu Zhang; Zhiheng Yang; Chuan Li; Qian Zhang; Tong Shi; Liming Zhou; Feng Xie; Huanqin Dai; Xueting Liu; Jingyu Zhang; Guang Liu; Ying Zhuo; Buchang Zhang; Chenli Liu; Shanshan Li; Xuekui Xia; Yaojun Tong; Yanwen Liu; Gil Alterovitz; Gao-Yi Tan; Li-Xin Zhang
• Keywords: High-Throughput Screening; Biomolecule

Abstract Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it not the of small molecules. Bacterial allosteric transcription factors (aTFs) have evolved sense and respond sensitively a variety molecules benefit bacterial survival. By combining single-stranded cleavage ability competitive binding activities aTFs double-stranded DNA, here we develop simple, supersensitive, fast high-throughput platform molecules, designated CaT-SMelor ( C RISPR-Cas12a- aT F-mediated s mall m ol e cu l detect or ). is successfully evaluated by detecting nanomolar levels various including uric acid p -hydroxybenzoic among their structurally similar analogues. We also demonstrate that directly measured concentration in clinical human blood samples, indicating great potential