Mapping optogenetically-driven single-vessel fMRI with concurrent neuronal calcium recordings in the rat hippocampus

• DOI: 10.1038/s41467-019-12850-x
• Publication Date: 2019-11-20T11:04:02Z
• Authors: Xuming Chen; Filip Sobczak; Yi Chen; Yuanyuan Jiang; Chunqi Qian; Zuneng Lu; Cenk Ayata; Nikos K. Logothetis; Xin Yu
• Keywords: Calcium imaging; Cortical Spreading Depression; Haemodynamic response; Premovement neuronal activity; Brain mapping

Abstract Extensive in vivo imaging studies investigate the hippocampal neural network function, mainly focusing on dorsal CA1 region given its optical accessibility. Multi-modality fMRI with simultaneous electrophysiological recording reveal broad cortical correlation patterns, but detailed spatial functional map remains lacking limited resolution. In particular, hemodynamic responses linked to specific activity are unclear at single-vessel level across vasculature, which hinders deciphering of malfunction animal models and translation critical neurovascular coupling (NVC) patterns for human fMRI. We simultaneously acquired optogenetically-driven neuronal Ca 2+ signals blood-oxygen-level-dependent (BOLD) cerebral-blood-volume (CBV)-fMRI from individual venules arterioles. Distinct spatiotemporal were correlated optogenetically evoked spreading depression-like calcium events. The event-related modeling revealed significantly reduced NVC efficiency upon (SDL) events, providing a direct measure function various states.