Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation

• DOI: 10.1038/s41467-019-13382-0
• Publication Date: 2019-11-29T11:03:10Z
• Authors: Ayshwarya Subrama...; Eriene-Heidi Sidhom; Maheswarareddy Emani; Katherine Vernon; Nareh Sahakian; Yiming Zhou; Maria Kost-Alimova; Michal Slyper; Julia Waldman; Danielle Dionne; Lan T. Nguyen; Astrid Weins; Jamie L. Marshall; Orit Rosenblatt-R...; Aviv Regev; Anna Greka
• Keywords: Organoid

Abstract Human iPSC-derived kidney organoids have the potential to revolutionize discovery, but assessing their consistency and reproducibility across iPSC lines, reducing generation of off-target cells remain an open challenge. Here, we profile four human lines for a total 450,118 single show how organoid composition development are comparable fetal adult kidneys. Although cell classes largely reproducible time points, protocols, replicates, detect variability in proportions between different due cells. To address this, analyze transplanted under mouse capsule find diminished Our work shows RNA-seq (scRNA-seq) can score reproducibility, faithfulness quality, that derived from surrogates kidney, transplantation enhances formation by diminishing