A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism

• DOI: 10.1038/s41467-020-16504-1
• Publication Date: 2020-05-29T10:02:27Z
• Authors: J. J. David Ho; Nathan C. Balukoff; Phaedra R. Theodo...; Miling Wang; Jonathan R. Krieger; Jonathan H. Schatz; Stephen Lee
• Keywords: Proteome; Translational efficiency; Translational regulation

Abstract Protein expression evolves under greater evolutionary constraint than mRNA levels, and translation efficiency represents a primary determinant of protein levels during stimuli adaptation. This raises the question as to translatome remodelers that titrate output from populations. Here, we uncover network RNA-binding proteins (RBPs) enhances glycolytic in cells responding oxygen deprivation. A system-wide proteomic survey translational engagement identifies family oxygen-regulated RBPs functions switch intensity. Tandem mass tag-pulse SILAC (TMT-pSILAC) RNA sequencing reveals each RBP controls unique but overlapping portfolio hypoxic responsive proteins. These collaborate with synthesis apparatus, operating checkpoint integrates upstream signals activate anaerobic metabolism. system allows anoxia-resistant animals mammalian initiate glycolysis survive hypoxia. We suggest an oxygen-sensitive cluster metabolism confer hypoxia tolerance.