FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato

• DOI: 10.1038/s41467-020-19705-w
• Publication Date: 2020-11-17T11:03:31Z
• Authors: Ren Li; Shuai Sun; Haijing Wang; Ketao Wang; Hong Yu; Zhen Zhou; Peiyong Xin; Jinfang Chu; Tongmin Zhao; Huanzhong Wang; Jiayang Li; Xia Cui
• Keywords: Gene Editing; 2. Zero hunger; 0303 health sciences; Science; Q; Quantitative Trait Loci; Plants, Genetically Modified; Article; Gibberellins; Dioxygenases; Gene Knockout Techniques; Plant Breeding; 03 medical and health sciences; Solanum lycopersicum; Gene Expression Regulation, Plant; Fruit; Mutation; Plant Physiological Phenomena; Plant Proteins

Abstract Fruit firmness is a target trait in tomato breeding because it facilitates transportation and storage. However, also complex uncovering the molecular genetic mechanisms controlling fruit has proven challenging. Here, we report map-based cloning functional characterization of qFIRM SKIN 1 ( qFIS1 ), major quantitative locus that partially determines difference compression resistance between cultivated wild accessions. FIS1 encodes GA2-oxidase, its mutation leads to increased bioactive gibberellin content, enhanced cutin wax biosynthesis, shelf life. Importantly, no unfavorable effect on weight or taste, making an ideal for breeders. Our study demonstrates mediates catabolism regulates firmness, offers potential strategy breeders produce firmer fruit.