A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

• DOI: 10.1038/s41467-021-23738-0
• Publication Date: 2021-06-07T10:20:51Z
• Authors: Jianping Zhang; Timothy C. Hewitt; Willem H. P. Boshoff; Ian Dundas; Narayana Upadhyaya; Jianbo Li; Mehran Patpour; Sutha Chandramohan; Zacharias A. Pret...; Mogens Hovmøller; Wendelin Schnippe...; Robert F. Park; Rohit Mago; Sambasivam Periya...; Dhara Bhatt; Sami Hoxha; Soma Chakraborty; Ming Luo; Peter Dodds; Burkhard Steuernagel; Brande B. H. Wulff; Michael Ayliffe; Robert A. McIntosh; Peng Zhang; Evans S. Lagudah
• Keywords: Comparative Genomics

Abstract The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 Sr61 , with both genes independently introduced as alien chromosome introgressions tall grass ( Thinopyrum ponticum ). Mutational genomics targeted exome capture identify separate single that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. orthologs absent current elongatum pan genome sequences, contrasting where homologues present. Using gene-specific markers, validate presence a recombinant segment developed wheat. co-location these small non-recombinogenic simplifies their deployment stack potentially enhances durability.