LC3B is an RNA-binding protein to trigger rapid mRNA degradation during autophagy

• DOI: 10.1038/s41467-022-29139-1
• Publication Date: 2022-03-17T11:04:45Z
• Authors: Hyun Jung Hwang; Hongseok Ha; Ban Seok Lee; Bong Heon Kim; Hyun Kyu Song; Yoon Ki Kim
• Keywords: ATG8

Abstract LC3/ATG8 has long been appreciated to play a central role in autophagy, by which variety of cytoplasmic materials are delivered lysosomes and eventually degraded. However, information on the molecular functions LC3 RNA biology is very limited. Here, we show that LC3B an RNA-binding protein directly binds mRNAs with preference for consensus AAUAAA motif corresponding polyadenylation sequence. Autophagic activation promotes association between target triggers rapid degradation CCR4-NOT–dependent manner before autolysosome formation. Furthermore, our transcriptome-wide analysis reveals PRMT1 mRNA, encodes negative regulator one major substrates. Rapid mRNA facilitates autophagy. Collectively, demonstrate acts as decay factor necessary efficient