Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms

• DOI: 10.1038/s41467-022-29228-1
• Publication Date: 2022-03-21T11:03:30Z
• Authors: Piaopiao Chen; Agnès H. Michel; Jianzhi Zhang
• Keywords: Insertional mutagenesis; Transposon mutagenesis; DNA Transposable Elements

Abstract Due to epistasis, the same mutation can have drastically different phenotypic consequences in individuals. This phenomenon is pertinent precision medicine as well antimicrobial drug development, but its general characteristics are largely unknown. We approach this question by genome-wide assessment of gene essentiality polymorphism 16 Saccharomyces cerevisiae strains using transposon insertional mutagenesis. Essentiality observed for 9.8% genes, most which had repeated switches evolution. Genes exhibiting lean toward having intermediate numbers genetic and protein interactions. Gene changes tend occur concordantly among components complex or metabolic pathway a group over 100 mitochondrial proteins, revealing molecular machines functional modules units variation. Most essential genes tolerate insertions consistently one more coding segments, delineating nonessential regions within genes.