Multiomic profiling of the acute stress response in the mouse hippocampus

• DOI: 10.1038/s41467-022-29367-5
• Publication Date: 2022-04-05T10:12:26Z
• Authors: Lukas M. von Ziegler; Amalia Floriou-Se...; Rebecca Waag; Rebecca R. Das Gupta; Oliver Sturman; Katharina Gapp; Christina A. Maat; Tobias Kockmann; Han-Yu Lin; Sian N. Duss; Mattia Privitera; Laura Hinte; Ferdinand von Meyenn; Hanns U. Zeilhofer; Pierre-Luc Germain; Johannes Bohacek
• Keywords: Integrated stress response; Stress granule

Abstract The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. However, the underlying molecular cascades are unclear. Here, we use a brief swim exposure trigger an in mice, which transiently increases anxiety, without leading lasting maladaptive changes. Using multiomic profiling, such as proteomics, phospho-proteomics, bulk mRNA-, single-nuclei small RNA-, TRAP-sequencing, characterize stress-induced events mouse hippocampus over time. Our results show complexity specificity of stress, highlighting both widespread changes protein phosphorylation gene transcription, tightly regulated translation. observed resolve efficiently within four hours after initiation stress. We include interactive app explore data, providing resource that can help us understand how impacts brain function