Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae

• DOI: 10.1038/s41467-022-29560-6
• Publication Date: 2022-04-07T10:20:54Z
• Authors: Hui Liu; Pei Zhou; Mengya Qi; Liang Guo; Cong Gao; Guipeng Hu; Wei Song; Jing Wu; Xiulai Chen; Jian Chen; Wei Chen; Liming Liu
• Keywords: 0303 health sciences; Science; Q; Fatty Acids; Saccharomyces cerevisiae; 7. Clean energy; Article; Actins; Actin Cytoskeleton; 03 medical and health sciences; 1-Butanol; Metabolic Engineering; Biofuels

Abstract Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer actin cytoskeleton S. to increase both growth and n-butanol medium-chain fatty acids. Actin cable tortuosity regulated using an responsive promoter-based autonomous bidirectional signal conditioner . The budding index increased by 14.0%, resulting highest titer 1674.3 mg L −1 Moreover, patch density fine-tuned acid conditioner. intracellular pH stabilized at 6.4, yielding acids 692.3 yeast extract peptone dextrose medium. Engineering can efficiently alleviate biofuels enhance