Differences in RNA polymerase II complexes and their interactions with surrounding chromatin on human and cytomegalovirus genomes

• DOI: 10.1038/s41467-022-29739-x
• Publication Date: 2022-04-14T10:02:54Z
• Authors: Benjamin M. Spector; Mrutyunjaya Parida; Ming Li; Christopher B. Ball; Jeffery L. Meier; Donal S. Luse; David H. Price
• Keywords: RNA polymerase II; Transcription preinitiation complex; Chromatin immunoprecipitation

Abstract Interactions of the RNA polymerase II (Pol II) preinitiation complex (PIC) and paused early elongation complexes with first downstream (+1) nucleosome are thought to be functionally important. However, current methods limited for investigating these relationships, both cellular chromatin human cytomegalovirus (HCMV) genome. Digestion DNA fragmentation factor (DFF) before immunoprecipitation (DFF-ChIP) precisely revealed similarities major differences in PICs driven by TBP on host genome comparison or viral-specific, late initiation UL87 viral Host Pol frequently found contact +1 can also a involved initial invasion nucleosome. In contrast, transcription have very nucleosomal interactions, reflecting relative lack chromatinization transcriptionally active regions HCMV genomes.