HnRNPK maintains single strand RNA through controlling double-strand RNA in mammalian cells

• DOI: 10.1038/s41467-022-32537-0
• Publication Date: 2022-08-29T10:03:45Z
• Authors: Sagar Mahale; Meenakshi Setia; Bharat Prajapati; Santhilal Subhash; Mukesh Pratap Yadav; Subazini Thankasw...; Ananya Deshpande; Jagannath Kuchlyan; Mirco Di Marco; Fredrik Westerlund; L. Marcus Wilhelm...; Chandrasekhar Kan...; Meena Kanduri
• Keywords: RNA Silencing; Transcription

Abstract Although antisense transcription is a widespread event in the mammalian genome, double-stranded RNA (dsRNA) formation between sense and transcripts very rare mechanisms that control dsRNA remain unknown. By characterizing FGF-2 regulated transcriptome normal cancer cells, we identified IER3 IER3-AS1 play critical role controlled oncogenic pathways. We show regulate each other’s through HnRNPK-mediated post-transcriptional regulation. HnRNPK controls mRNA stability colocalization of IER3-AS1. interaction with determines their functions by maintaining them single-stranded form. hnRNPK depletion neutralizes promoting cytoplasmic accumulation. Intriguingly, loss-of-function gain-of-function experiments reveal its global single- RNA. Thus, our data unveil RNAs physiological blocking RNA-RNA interactions.