A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias

• DOI: 10.1038/s41467-022-34739-y
• Publication Date: 2022-11-23T00:11:54Z
• Authors: Sebald A. N. Verk...; Estela Gonzalez; Ming Li; Joshua X. D. Ang; Nikolay P. Kandul; Michelle A. E. An...; Omar S. Akbari; Michael B. Bonsall; Luke Alphey
• Keywords: Homing endonuclease; Gene drive

Abstract CRISPR/Cas gene drives can bias transgene inheritance through different mechanisms. Homing are designed to replace a wild-type allele with copy of drive element on the homologous chromosome. In Aedes aegypti , sex-determining locus is closely linked white gene, which was previously used as target for homing ( w GDe ). Here, an analysis using this linkage we show that in males did not occur by homing, rather increased propagation donor element. We test same transgenes expressing Cas9 germline regulatory elements sds3 bgcn and nup50 . only find even identical -Cas9 transgene. propose DNA repair outcomes may be more context dependent than anticipated other reported may, fact, their