CRY2 interacts with CIS1 to regulate thermosensory flowering via FLM alternative splicing

• DOI: 10.1038/s41467-022-34886-2
• Publication Date: 2022-11-17T21:02:36Z
• Authors: Zhiwei Zhao; Craig Dent; Huafeng Liang; Junqing Lv; Guandong Shang; Yawen Liu; Fan Feng; Fei Wang; Junhong Pang; Xu Li; Libang Ma; Bing Li; Sridevi Sureshkumar; Jia-Wei Wang; Sureshkumar Balas...; Hongtao Liu
• Keywords: 0303 health sciences; Arabidopsis Proteins; Science; Q; Arabidopsis; MADS Domain Proteins; Flowers; Article; Cryptochromes; Alternative Splicing; 03 medical and health sciences; Gene Expression Regulation, Plant; RNA Precursors; Transcription Factors

Abstract Cryptochromes (CRYs) are evolutionarily conserved photolyase-like photoreceptors found in almost all species, including mammals. CRYs regulate transcription by modulating the activity of several factors, but whether and how they affect pre-mRNA processing unknown. Photoperiod temperature closely associated seasonal cues that influence reproductive timing plants. mediate photoperiod-responsive floral initiation, it is largely unknown also involved thermosensory flowering. We establish here blue light CRY2 play critical roles flowering Arabidopsis thaliana regulating RNA alternative splicing (AS) to protein expression development. INTERACTING SPLICING FACTOR 1 (CIS1) interacts with a light–dependent manner promotes CRY2–mediated Blue light, CRYs, CISs transcriptome-wide AS profiles, those FLOWERING LOCUS M ( FLM ), which for modulation Moreover, CIS1 binds its AS, while regulates RNA-binding CIS1. Thus, via CRY2–CIS1–FLM signaling pathway links responses both ambient temperature.