Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin

• DOI: 10.1038/s41467-022-34908-z
• Publication Date: 2022-11-22T22:02:40Z
• Authors: Zhuozhi Chen; Rongdi Duan; Yunjie Xiao; Yi Wei; Hanxiao Zhang; Xinzhao Sun; Shen Wang; Yingying Cheng; Xue Wang; Shanwei Tong; Yunxiao Yao; Cheng Zhu; Haitao Yang; Yanyan Wang; Zefang Wang
• Keywords: Hydrophobin; Degradation

The process of recycling poly(ethylene terephthalate) (PET) remains a major challenge due to the enzymatic degradation high-crystallinity PET (hcPET). Recently, bacterial PET-degrading enzyme, PETase, was found have ability degrade hcPET, but with low activity. Here we present an engineered whole-cell biocatalyst simulate both adsorption and steps in PETase achieve efficient hcPET. Our data shows that adhesive unit hydrophobin are functionally displayed on surface yeast cells. turnover rate toward hcPET (crystallinity 45%) dramatically increases approximately 328.8-fold compared purified at 30 °C. In addition, molecular dynamics simulations explain how enhanced adhesion can promote PET. This study demonstrates engineering catalyst is strategy for biodegradation