Early response evaluation by single cell signaling profiling in acute myeloid leukemia

• DOI: 10.1038/s41467-022-35624-4
• Publication Date: 2023-01-07T15:24:34Z
• Authors: Benedicte Sjo Tis...; Monica Hellesøy; Oda Helen Eck Fag...; Stein-Erik Gullaksen; Aashish Srivastava; Even Birkeland; Dimitrios Kleftog...; Pilar Ayuda-Durán; Laure Piechaczyk; Dagim Shiferaw Ta...; Jørn Skavland; Panagotis Baliakas; Randi Hovland; Vibeke Andresen; Ole Morten Seternes; Tor Henrik Anders...; Nima Aghaeepour; Sonia Gavasso; Kimmo Porkka; Inge Jonassen; Yngvar Fløisand; Jorrit Enserink; Nello Blaser; Bjørn Tore Gjertsen
• Keywords: Mass cytometry; Induction chemotherapy

Aberrant pro-survival signaling is a hallmark of cancer cells, but the response to chemotherapy poorly understood. In this study, we investigate initial standard induction in cohort 32 acute myeloid leukemia (AML) patients, using 36-dimensional mass cytometry. Through supervised and unsupervised machine learning approaches, find that reduction extracellular-signal-regulated kinase (ERK) 1/2 p38 mitogen-activated protein (MAPK) phosphorylation cell compartment 24 h post-chemotherapy significant predictor patient 5-year overall survival cohort. Validation by RNA sequencing shows MAPK target gene expression patients with high phospho-ERK1/2 post-chemotherapy, while proteomics confirm an increase prime activated 2 (MAPKAPK2). demonstrate cytometry can be valuable tool for early evaluation AML elucidate potential functional analyses precision oncology diagnostics.