Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana

• DOI: 10.1038/s41467-024-50143-0
• Publication Date: 2024-07-12T20:24:10Z
• Authors: Sara C. Pinto; Weng Herng Leong; Hweiting Tan; Lauren McKee; Amelie Prevost; Chao Ma; Neil. J. Shirley; Rosanna Petrella; Xiujuan Yang; Anna M. Koltunow; Vincent Bulone; Masahiro M. Kanaoka; Tetsuya Higashyiama; Sílvia Coimbra; Matthew R. Tucker
• Keywords: Ovule; Gametogenesis, Plant; 0301 basic medicine; beta-Glucans; Arabidopsis Proteins; Science; Gene Expression Profiling; Q; Arabidopsis; Plasmodesmata; Article; 03 medical and health sciences; Gene Expression Regulation, Plant; Pollen

Abstract Correct regulation of intercellular communication is a fundamental requirement for cell differentiation. In Arabidopsis thaliana , the female germline differentiates from single somatic ovule that becomes encased in β−1,3-glucan, water insoluble polysaccharide implicated limiting pathogen invasion, regulating trafficking roots, and promoting pollen development. Whether β−1,3-glucan facilitates isolation development has remained contentious, since limited evidence available to support functional role. Here, transcriptional profiling adjoining cells revealed differences gene expression related metabolism signalling through channels (plasmodesmata). Dominant β−1,3-glucanase transiently perturbed deposits, allowed movement tracer molecules, led changes histone marks, eventually leading termination Our findings indicate fulfils role by insulating primary cell, thereby determines success downstream gametogenesis.