The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3

• DOI: 10.1038/s41467-024-53277-3
• Publication Date: 2024-10-15T14:43:21Z
• Authors: Zhuo Han; Rui Wang; Pengliang Chi; Zihan Zhang; Ling Min; Haizhan Jiao; Guojin Ou; Dan Zhou; Dandan Qin; Chengpeng Xu; Zheng Gao; Qianqian Qi; Jialu Li; Yuechao Lu; Xiang Wang; Jing Chen; Xingjiang Yu; Hongli Hu; Lei Li; Dong Deng
• Keywords: Zygote; Science; Q; Cell Cycle; Embryonic Development; Embryo, Mammalian; Article; Mice; 14-3-3 Proteins; Oocytes; Animals; Humans; cdc25 Phosphatases; Female; Phosphorylation

The subcortical maternal complex (SCMC) is essential for safeguarding female fertility in mammals. Assembled oocytes, the SCMC maintains cleavage of early embryos, but underlying mechanism remains unclear. Here, we report that 14-3-3, a multifunctional protein, component SCMC. By resolving structure 14-3-3-containing SCMC, discover phosphorylation TLE6 contributes to recruitment 14-3-3. Mechanistically, during maternal-to-embryo transition, stabilizes 14-3-3 protein and proper control CDC25B, thus ensuring activation maturation-promoting factor mitotic entry mouse zygotes. Notably, establishes conserved molecular link with CDC25B human oocytes/embryos. This study discloses through which regulates cell cycle embryos elucidates function mammalian embryogenesis.