Vascular FLRT2 regulates venous-mediated angiogenic expansion and CNS barriergenesis

• DOI: 10.1038/s41467-024-54570-x
• Publication Date: 2024-11-29T03:21:38Z
• Authors: C. Llaó-Cid; B. Peguera; P. Kobialka; L. Decker; J. Vogenstahl; N. Alivodej; S. Srivastava; J. Jin; B. C. Kirchmaier; C. Milla; H. Schlierbach; A. Schänzer; T. Acker; M. Segarra; A. Acker-Palmer
• Keywords: Adherens junction; VE-cadherin; Sprouting angiogenesis

Abstract Veins have emerged as the origin of all other endothelial cell subtypes needed to expand vascular networks during developmental and pathological neoangiogenesis. Here, we uncover role angioneurin Fibronectin Leucine Rich Transmembrane protein (FLRT) 2 in central nervous system (CNS) development mouse. Early postnatal FLRT2 deletion reveals specific defects retinal veins, impacting proliferation, sprouting polarity that result reduced tip cells at front. interacts with VE-cadherin together endocytic adaptor Numb contribute modulation adherens junction morphology both retina cerebral cortex vivo. Utilizing expansion microscopy, visualize altered dynamic distribution tissue mutants. Additionally, cortical vessels regulates crosstalk between tight junctions, influencing blood-brain barrier development. Our findings position a vein-specific regulator CNS