Random-access wide-field mesoscopy for centimetre-scale imaging of biodynamics with subcellular resolution

• DOI: 10.1038/s41566-024-01422-1
• Publication Date: 2024-04-17T10:01:58Z
• Authors: Ruheng Shi; Xinyue Chen; Junhao Deng; Junhao Liang; Kuikui Fan; Feifan Zhou; Peifu Tang; Licheng Zhang; Lingjie Kong
• Keywords: Field of view; Random access

Abstract Benefitting from the advantages of high imaging throughput and low cost, wide-field microscopy has become indispensable in biomedical studies. However, it remains challenging to record biodynamics with a large field view spatiotemporal resolution due limited space–bandwidth product. Here we propose random-access (RA-WiFi) mesoscopy for vivo over 163.84 mm 2 area spatial ~2.18 μm. We extend beyond nominal value objective by enlarging object distance, which leads lower angle, followed correction optical aberrations. also implement scanning structured illumination, enables optical-sectioning capability contrast. The multi-plane makes technique suitable curved-surface samples. demonstrate RA-WiFi multi-modal imaging, including bright-field, dark-field multi-colour fluorescence imaging. Specifically, apply calcium cortex-wide neural network activities awake mice vivo, under both physiological pathological conditions. show its unique three-dimensional random access irregular regions interest via biodynamic mouse spinal cords vivo. As compact, low-cost mesoscope capability, will enable broad applications study biological systems.