Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides

• DOI: 10.1038/s41598-017-07516-x
• Publication Date: 2017-07-26T12:57:44Z
• Authors: Fenglong Jiao; Fangyuan Gao; Heping Wang; Yulin Deng; Yangjun Zhang; Xiaohong Qian; Yukui Zhang
• Keywords: HeLa; Exosome

The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing excellent hydrophilicity and strong electrostatic interaction toward polymerized liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), Fe3O4@MPS@PMAC exhibited outstanding performance glycopeptide enrichment with high detection sensitivity (10 fmol), large binding capacity (100 μg mg-1) satisfied recovery (approximately 82%). Furthermore, newly developed applied for HeLa exosome proteins. A total 1274 from 536 glycoproteins identified three replicate analyses 50 These results demonstrate potential both glycoproteomic analysis research.