Characterization of a murine model of non-lethal, symptomatic dengue virus infection

• DOI: 10.1038/s41598-018-22618-w
• Publication Date: 2018-03-14T15:01:26Z
• Authors: Vanessa V. Sarathy; Mellodee White; Li Li; Jaclyn A. Kaiser; Gerald A. Campbell; Gregg N. Milligan; Nigel Bourne; Alan D. T. Barrett
• Keywords: Male; 0301 basic medicine; Mice, 129 Strain; Genotype; Mice, Transgenic; Dengue Virus; Disseminated Intravascular Coagulation; Serogroup; Thrombocytopenia; Article; 3. Good health; Dengue; Disease Models, Animal; Immunocompromised Host; Mice; 03 medical and health sciences; Animals; Cytokines; Humans; RNA, Viral; Female; Receptors, Interferon

Abstract The mosquito-borne disease dengue is caused by four serologically- and genetically-related viruses, termed DENV-1 to DENV-4. Historical setbacks due lack of human-like mouse models were partially remedied with characterization lethal DENV-2 infection in immunocompromised AG129 mice (deficient IFN-α/β/γ receptors). Recently, our group established DENV-1, DENV-3, DENV-4 using human isolates. Here we compare a non-lethal, disseminated model DENV-3 strain D83-144 that the outcome following C0360/94. Both strains belong genotype II differ only 13 amino acids. Intraperitoneal inoculation led clinical signs infection, such as cytokine induction, thrombocytopenia, systemic infection. However, C0360/94 features severe dengue, including coagulopathy outcome, whereas does not. This study first investigate low passage, non-mouse demonstrates induces milder than those induced results suggest has applications factors associated mild or disease.