Genome plasticity is governed by double strand break DNA repair in Streptomyces

• DOI: 10.1038/s41598-018-23622-w
• Publication Date: 2018-03-29T02:05:50Z
• Authors: Grégory Hoff; Claire Bertrand; Emilie Piotrowski; Annabelle Thibessard; Pierre Leblond
• Keywords: cassure chromosomique; DNA, Bacterial; DNA End-Joining Repair; 572; DNA Repair; Article; Evolution, Molecular; 03 medical and health sciences; [SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]; DNA Breaks, Double-Stranded; streptomyces; réparation de l'adn; coupure double brin; 0303 health sciences; Base Sequence; [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]; plasticité; génome; Chromosomes, Bacterial; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; Streptomyces; Mutagenesis; adn chromosomique; [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; Genome, Bacterial

Abstract The linear chromosome of the bacterium Streptomyces exhibits a remarkable genetic organization with grossly central conserved region flanked by variable chromosomal arms. terminal diversity co-locates an intense DNA plasticity including occurrence large deletions associated to circularization and arm exchange. These observations prompted us assess role double strand break (DSB) repair in following. For that purpose, DSBs were induced along using meganuclease I- Sce I. DSB was mutagenic at healing site but kept intact whole genome structure. In contrast, arms mostly loss targeted extensive beyond cleavage sites. While homologous recombination occurring between copies sequences accounted for most part rescue events, Non Homologous End Joining involved as well huge rearrangements (i.e. circularization). Further, NHEJ concomitant integration material site. We postulate drives evolution may foster horizontal transfer environment.