Analysis of population structures of the microalga Acutodesmus obliquus during lipid production using multi-dimensional single-cell analysis

• DOI: 10.1038/s41598-018-24638-y
• Publication Date: 2018-04-13T09:21:34Z
• Authors: Michael Sandmann; Michaela Schafberg; Martin Lippold; Sascha Rohn
• Keywords: Scenedesmus obliquus; Single-Cell Analysis

Microalgae bear a great potential to produce lipids for biodiesel, feed, or even food applications. To understand the still not well-known single-cell dynamics during lipid production in microalgae, novel analytical technology was applied study well-established model experiment. Multidimensional were investigated with non-supervised image analysis technique that utilizes data from epi-fluorescence microscopy. Reliability of this successfully proven via reference analysis. The developed used determine cell size, chlorophyll amount, neutral and deriving properties on single-cellular level cultures biotechnologically promising alga Acutodesmus obliquus. results illustrated high correlation between size but very low dynamic density. During growth conditions under nitrogen starvation, cells content tend start first suspension differentiated two subpopulations significantly different contents. Such quantitative characterization synthesizing algae done time such simple is highly relevant other biotechnological applications deeper investigate process microalgal accumulation.