Label-free and Non-destruction Determination of Single- and Double-Strand DNA based on Quantum Weak Measurement

• DOI: 10.1038/s41598-018-38454-x
• Publication Date: 2019-02-13T11:05:13Z
• Authors: Tian Guan; Yuxuan Yang; Qianwen Zhang; Yonghong He; Naihan Xu; Dongmei Li; Lixuan Shi; Yang Xu; Xiangnan Wang
• Keywords: Base Sequence; Nucleic Acid Renaturation; Temperature; DNA, Single-Stranded; Quantum Theory; DNA; Hydrogen-Ion Concentration; 01 natural sciences; Article; 0104 chemical sciences

Abstract The process of unwinding and renaturation DNA has been widely used in studies nucleotide sequence organization. Compared with traditional methods for renaturation, the label-free non-destruction detection technology is significant desiderated. We realized an optical system based on rotation via weak measurement single- double-strand state DNA. rotation, which was induced by status change single double strands, exploited to modulate preselected polarization a system. With this modulation, caused separation strands can be determined through center wavelength shift output spectrum. By monitoring real time, processes DNAs different base ratio (25% 70%) length (4nt 40nt), three terminally modified cholesterol molecules were experimentally explored varied pH temperature conditions. In addition, limit concentration obtained 5 × 10 −6 mol/L. Our work exhibits unique advantages real-time monitoring, label-free, simplicity.