Genome-wide characterization of bZIP gene family identifies potential members involved in flavonoids biosynthesis in Ginkgo biloba L.

• DOI: 10.1038/s41598-021-02839-2
• Publication Date: 2021-12-03T11:03:41Z
• Authors: Huan Han; Feng Xu; Yuting Li; Li Yu; Mingyue Fu; Yongling Liao; Xiaoyan Yang; Weiwei Zhang; Jiabao Ye
• Keywords: Flavonoids; 2. Zero hunger; 0301 basic medicine; Science; Gene Expression Profiling; Q; R; Computational Biology; Ginkgo biloba; Germination; Article; Cold Temperature; Plant Leaves; 03 medical and health sciences; Basic-Leucine Zipper Transcription Factors; Gene Expression Regulation, Plant; Medicine; Computer Simulation; Tissue Distribution; Genome, Plant; Phylogeny; Plant Proteins

Ginkgo biloba L. is an ancient relict plant with rich pharmacological activity and nutritional value, its main physiologically active components are flavonoids terpene lactones. The bZIP gene family one of the largest families in plants regulates many processes including pathogen defense, secondary metabolism, stress response, seed maturation, flower development. In this study, genome-wide distribution transcription factors was screened from G. database silico analysis. A total 40 genes were identified divided into 10 subclasses. GbbZIP members same group share a similar structure, number introns exons, motif distribution. Analysis tissue expression pattern based on transcriptome indicated that GbbZIP08 GbbZIP15 most highly expressed mature leaf. And level GbbZIP13 high all eight tissues. Correlation analysis phylogenetic tree suggested might be involved flavonoid biosynthesis. transcriptional levels 20 after SA, MeJA, low temperature treatment analyzed by qRT-PCR. significantly upregulated under 4°C. Protein-protein interaction network GbbZIP09 participate germination interacting GbbZIP32. Based degradome data, we found 32 out 117 miRNAs annotated to 17 miRNA families. results study may provide theoretical foundation for functional validation future.