Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn

• DOI: 10.1038/s41598-021-03472-9
• Publication Date: 2021-12-21T11:03:53Z
• Authors: Marta Oliveira; Caroline Sprengel...; Setha Ketavong; Eulogio J. Lloren...; Hervé Hoste; Luísa Custódio
• Keywords: Nutraceutical

Abstract Strategies to reduce dependence on synthetic drugs for the treatment of gastrointestinal nematodes (GIN) infections in ruminants include search novel anthelmintic scaffolds plants, yet salt-tolerant plants remain overlooked. This study aims evaluate vitro properties selected against GIN, and identify potential bioactive secondary metabolites involved. For that purpose, 80% acetone/water extracts were prepared from dried biomass aerial organs nine plant species tested Haemonchus contortus Trichostrongylus colubriformis by Larval Exsheathment Inhibition Assay (LEIA) Egg Hatching (EHIA). Pistacia lentiscus, Limoniatrum monopetalum, Cladium mariscus Helychrisum italicum picardi most active both GIN life stages. To investigate role polyphenols activity, four treated with polyvinylpolypyrrolidone (PVPP), non-treated samples further characterized high-performance liquid chromatography electrospray ionization mass spectrometric detection (HPLC-ESI-MS n ). While seem responsible EHIA properties, they are partially accountable LEIA results. Several phenolics involved effects identified discussed. In sum, these rich sources compounds and, therefore, major interest nutraceutical and/or phytotherapeutic applications ruminants.