Altered high-density lipoprotein composition and functions during severe COVID-19

• DOI: 10.1038/s41598-021-81638-1
• Publication Date: 2021-01-27T11:06:38Z
• Authors: Floran Begue; Sébastien Tanaka; Zarouki Mouktadi; Philippe Rondeau; Bryan Veeren; Nicolas Diotel; Alexy Tran-Dinh; Tiphaine Robert; Erick Vélia; Patrick Mavingui; Marie Lagrange-Xélot; Philippe Montravers; David Couret; Olivier Meilhac
• Keywords: Paraoxonase; Proteome; High-density lipoprotein

Abstract Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing proteome functionality during relative healthy subjects. HDLs isolated from plasma 8 severe sampled admission intensive care unit (Day 1, D1) D3 D7, 16 sex- age-matched Proteomic analysis was performed by LC-MS/MS. amounts proteins identified compared between controls. apolipoprotein A-I paraoxonase 1 confirmed Western-blot be less abundant versus controls, whereas serum amyloid A alpha-1 antitrypsin higher. protective endothelial cells stiumalted TNFα (permeability, VE-cadherin disorganization apoptosis). In these conditions, inhibition apoptosis blunted conclusion, show major changes decreased