Zbp1-positive cells are osteogenic progenitors in periodontal ligament

• DOI: 10.1038/s41598-021-87016-1
• Publication Date: 2021-04-06T10:02:48Z
• Authors: Tsugumi Ueda; Tomoaki Iwayama; Kiwako Tomita; Shuji Matsumoto; Mizuho Iwashita; Phan Bhongsatiern; Hiromi Sakashita; Chiharu Fujihara; Masahide Takedachi; Shinya Murakami
• Keywords: Periodontal fiber

Periodontal ligament (PDL) possesses a stem/progenitor population to maintain the homeostasis of periodontal tissue. However, transcription factors that regulate this have not yet been identified. Thus, we aimed identify molecule related osteogenic differentiation PDL progenitors using single cell-based strategy in study. We first devised new protocol isolate cells from surface adult murine molars and established 35 cell-derived clones explant. Among these clones, six with high (high n = 3) low (low potential were selected. Despite clear difference no significant differences their cell morphology, progenitor marker expression, alkaline phosphatase activity, proliferation rate, differentiation-related gene protein expression observed. RNA-seq analysis revealed Z-DNA binding protein-1 (Zbp1) was significantly expressed indicating Zbp1 could be possible regulator cells. Zbp1-positive distributed sparsely throughout PDL. In vitro remained at level during differentiation. The CRISPR/Cas9 mediated knockout resulted delay On other hand, overexpression promoted These findings suggested marked Clarifying mechanism by future studies will facilitate better understanding tissue repair, possibly leading development novel therapeutic measures.