Inactivation of SARS-CoV-2 by a chitosan/α-Ag2WO4 composite generated by femtosecond laser irradiation

• DOI: 10.1038/s41598-022-11902-5
• Publication Date: 2022-05-17T10:09:46Z
• Authors: Paula Fabiana San...; Ana Carolina Alve...; Bruna Natália Alv...; Ivo Mateus Pinatti; Alexandre Zirpoli...; Carlos Eduardo Ve...; Débora Ferreira B...; Marcos Alexandre ...; Milene Dias Miranda; Maria Eduarda San...; Amanda Tucci; Carlos Doñate-Bue...; Gladys Mínguez-Vega; Juan Andrés; Elson Longo
• Keywords: 2019-20 coronavirus outbreak

In the current COVID-19 pandemic, next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent spread this virus within community. Herein, we report synthesis chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined estimating minimum inhibitory concentration (MIC) minimal bactericidal/fungicidal (MBC/MFC). To assess biocompatibility in a range involving MIC MBC/MFC on keratinocytes cells (NOK-si), an alamarBlue™ assay MTT were carried out. SARS-CoV-2 virucidal effects analyzed Vero E6 through viral titer quantified cell culture supernatant PFU/mL assay. Our results showed very similar 3.3 6.6, last one demonstrating slightly better action MSSA. 9.9 wide (0.49-31.25 µg/mL). cytotoxicity outcomes revealed that concentrations interest (MIC MBC/MFC) considered non-cytotoxic all after 72 h exposure. Chitosan/α-Ag2WO4 (CS6.6/α-Ag2WO4) composite reduced quantification up 80% controls. Then, our suggest these are highly efficient kill bacteria (Escherichia Methicillin-susceptible aureus, yeast strain albicans), addition inactivating contact, ROS production.