Comparing the extraction methods, chemical composition, phenolic contents and antioxidant activity of edible oils from Cannabis sativa and Silybum marianu seeds

• DOI: 10.1038/s41598-022-25030-7
• Publication Date: 2022-11-30T06:15:59Z
• Authors: Monika Kalinowska; Anna Płońska; Magdalena Trusiak; Ewelina Gołębiewska; Anna Gorlewska-Pi...
• Keywords: ABTS; Vanillic acid; Syringic acid; Protocatechuic acid

Abstract In the study cold-pressed, natural (unfiltered, unrefined) vegetable oils: hemp and milk thistle seed oils were tested for their chemical composition antioxidant properties. The physico-chemical parameters, content of saturated unsaturated fatty acids determined. Solid phase extraction simple with use methanol, ethanol, 80% ethanol used to obtain extracts analysis activity phenolic compounds in oils. was studied by means high-performance liquid chromatography (HPLC–DAD) spectrophotometric test Folin-Ciocalteu reagent. property established following methods: DPPH • (2,2-diphenyl-1-picrylhydrazyl) radical, ABTS •+ (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation FRAP (ferric ion reducing parameter) CUPRAC (cupric-reducing capacity). Moreover influence chlorogenic acid on inhibition lipid peroxidation process also investigated. showed different properties which related composition. main present oil vanillic, ferulic p -coumaric acids, (-)epicatechin, catechin, kaempferol procyanidin B2, whereas oil—catechins, C1, acid, phloridzin, quercetin, protocatechuic kaempferol, syringic acid. methanolic highest antiradical activity, ethanolic revealed best obtained parameters were: IC 50 = 3.433 ± 0.017 v/v (DPPH test), percent 93.301 1.099%, value 1063.883 39.225 µmol Fe 2+ , 420.471 1.765 Trolox. Whereas 5.280 0.584 79.59 3.763% (ABTS 2891.08 270.044 (FRAP 255.48 26.169 Trolox (CUPRAC assay). Chlorogenic effectively inhibited