Interplay of membrane crosslinking and curvature induction by annexins

• DOI: 10.1038/s41598-022-26633-w
• Publication Date: 2022-12-29T19:02:49Z
• Authors: Anna Mularski; Stine Lauritzen S...; Anne Sofie Busk H...; Mayank Prakash Pa...; Himanshu Khandelia; Jesper Nylandsted; Adam Cohen Simonsen
• Keywords: Annexin A2; Membrane Curvature; Annexin A1

Abstract Efficient plasma membrane repair (PMR) is required to damage sustained in the cellular life cycle. The annexin family of proteins, involved PMR, are activated by Ca 2+ influx from extracellular media at site injury. Mechanistic studies annexins have been overwhelmingly performed using a single annexin, despite recruitment multiple sites living cells. Hence, we investigate effect presence crosslinking annexins, A1, A2 and A6 (ANXA1, ANXA2 ANXA6) on curvature induction A4 (ANXA4) model systems. Our data support mechanistic PMR where ANXA4 induced ANXA6 promotes wound closure. now can be expanded include ANXA1 as specialist free edge crosslinkers that act concert with crosslinking.