Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time-resolved X-ray scattering

• DOI: 10.1038/s42003-018-0242-0
• Publication Date: 2018-12-27T14:54:39Z
• Authors: Derren J. Heyes; Samantha J. O. Ha...; Martin N. Pedersen; Joyce Woodhouse; Eugenio De La Mora; Michael Wulff; Martin Weik; Marco Cammarata; Nigel S. Scrutton; Giorgio Schirò
• Keywords: Phytochrome; Phototropism

Abstract Phytochromes are photoreceptor proteins that transmit a light signal from photosensory region to an output domain. Photoconversion involves protein conformational changes whose nature is not fully understood. Here, we use time-resolved X-ray scattering and optical spectroscopy study the kinetics of structural in full-length cyanobacterial phytochrome truncated form with no spectroscopic signals on µs/ms timescale largely independent presence On longer time-scales, large differences between indicate timeframe during which transition transmitted domain represent quaternary motion. The suggested independence photosensory-region dynamics defines time window photoreaction can be characterized (e.g. for optogenetic design) independently engineered