Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device

• DOI: 10.1038/s42003-019-0435-1
• Publication Date: 2019-05-20T10:03:37Z
• Authors: Zeinab Ramshani; Chenguang Zhang; Katherine Richards; Lulu Chen; Geyang Xu; Bangyan L. Stiles; Reginald Hill; Satyajyoti Senapati; David B. Go; Hsueh-Chia Chang
• Keywords: Extracellular Vesicles; Extracellular vesicles; Nanoparticle tracking analysis; Microfluidic chip

Abstract Extracellular vesicles (EV) containing microRNAs (miRNAs) have tremendous potential as biomarkers for the early detection of disease. Here, we present a simple and rapid PCR-free integrated microfluidics platform capable absolute quantification (<10% uncertainty) both free-floating miRNAs EV-miRNAs in plasma with 1 pM sensitivity. The assay time is only 30 minutes opposed to 13 h requires ~20 μL sample oppose mL conventional RT-qPCR techniques. integrates surface acoustic wave (SAW) EV lysing microfluidic chip concentration sensing incorporating an electrokinetic membrane sensor that based on non-equilibrium ionic currents. Unlike methods, this technology does not require extraction, RNA purification, reverse transcription, or amplification. This can be easily extended other DNA targets interest, thus providing viable screening tool disease diagnosis, prognosis, monitoring therapeutic response.